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. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Nature. 2017 Jan 4;541(7636):182–187. doi: 10.1038/nature21021

Extended Data Figure 1. Defining molecular domains in the developing stomach in vivo.

Extended Data Figure 1

a, Analysis of Sox2, Pdx1, and Gata4 in the embryonic mouse stomach (E14.5) showed that the fundus (f) was Sox2+Gata4+Pdx1−, whereas the antrum (a) was Sox2+Gata4+Pdx1+. The forestomach (fs) expressed Sox2 but neither Gata4 nor Pdx1. b, Brightfield stereomicrograph showing dissected regions of the E14.5 mouse stomach that were analyzed by qPCR. fs, forestomach; f, fundus; a, antrum; d, duodenum. c, Dissected regions in b were analyzed by qPCR for known regionally expressed markers (Sox2, P63, Gata4, Pdx1, and Cdx2) to validate the accuracy of micro-dissection. qPCR analysis of the dissected E14.5 stomach regions showed that putative fundus markers Irx1, Irx2, Irx3, Irx5, and Pitx1 were enriched in the fundus compared to the antrum. n=4 biological replicates per dissected region. Scale bar, 500 μm. Error bars represent s.d.