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. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Nature. 2017 Jan 4;541(7636):182–187. doi: 10.1038/nature21021

Figure 1. Wnt/β-catenin signaling is required for specification of the embryonic fundus in mice.

Figure 1

a, Pdx1 and Sox2 were expressed in the antrum (a), whereas Pdx1 was absent in the fundus (f), identified by Atp4b-expressing parietal cells at E18.5. b, X-gal staining of an E10.5 foregut from an Axin2:LacZ reporter embryo showed that Wnt activity was restricted to the anterior domain of the stomach but excluded from the posterior stomach. c, Deletion of β-catenin in the gastric epithelium caused an anterior expansion of Pdx1 into the fundic region of the stomach. d, In E18.5 Shh-cre;β-cateninfl/fl (cKO) embryos, Pdx1 was expressed throughout the stomach, except in some remaining patches of parietal cell-containing epithelium. Insets 1a-c and 2a-c show boxed regions in control and cKO stomach, respectively. e, In the cKO stomach, Ctnnb1 exhibited mosaic deletion, and parietal cells only differentiated in Ctnnb1-sufficient epithelium. Scale bars, 250 μm (a), 200 μm (c), and 500 μm (d and e).