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. Author manuscript; available in PMC: 2017 Jul 25.
Published in final edited form as: Nature. 2017 Jan 4;541(7636):182–187. doi: 10.1038/nature21021

Figure 4. Formation of chief cells in hFGOs.

Figure 4

a, hFGOs had a both MIST1 and Pepsinogen C (PGC) positive cells. b, High magnification of boxed region in panel (a) showing a gland with a cluster of cells with apical PGC staining. c, hFGOs had significantly increased expression of chief cell markers PGA5 (1,000-fold), PGC (100-fold), and MIST1 (>10-fold) as compared to hAGOs. **, p<0.05; two-tailed Student’s t-test. n=3 biological replicates, data representative of 4 independent experiments. d, Transmission electron micrograph of an hFGO cell containing dense zymogen granules, indicative of a chief cell. e, Pepsinogen protein content in hFGOs as compared to hAGOs in the presence or absence of the MEK inhibitor (PD03). **, p<0.0001 compared to hAGOs, two-tailed Student’s t-test, n=8, 12, and 11 biological replicates in hAGOs, control hFGOs and hFGOs (no PD03), respectively. Scale bars, 200 μm (a), 25 μm (b), and 10 μm (d). Error bars represent s.e.m.