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. Author manuscript; available in PMC: 2018 May 30.
Published in final edited form as: Cell Rep. 2017 May 30;19(9):1832–1845. doi: 10.1016/j.celrep.2017.05.021

Figure 3. TRIP13 knockdown causes a severely prolonged mitosis in Mad2-overexpressing cells.

Figure 3

(A) Schematic showing transfection of RPE-M p53 knockdown cells with TRIP13 or control siRNAs followed by doxycycline addition after 3 days followed by analysis by cell counting and time-lapse imaging. (B) Western blot of TRIP13 knockdown with two independent siRNAs with or without 24 hours of doxycycline treatment. Total quantification (endogenous + exogenous) is shown below corresponding bands. (C) Mitotic timing and fate of RPE-M cells transfected with control or TRIP13 siRNA +/- 24 hours doxycycline measured by cell rounding in time-lapse images. n ≥20 cells for each condition. *** indicates p < 0.001; Unpaired t test. Data are represented as mean± SD. See also Figures S1, S2, S3 and S4