| Antibodies |
| Anti-mouse CD117 conjugated to APC-Cy7 (clone 2B8) |
eBioscience |
Cat#47-1171-82 |
| Anti-mouse CD117 conjugated to APC (clone 2B8) |
eBioscience |
Cat#17-1171-82 |
| Anti-mouse CD11b conjugated to PE (clone M1/70) |
eBioscience |
Cat#12-0112-83 |
| Anti-mouse CD3 conjugated to PE (clone 145-2C11) |
eBioscience |
Cat#12-0031-85 |
| Anti-mouse CD4 conjugated to PE (clone RM4-5) |
eBioscience |
Cat#12-0042-85 |
| Anti-human/mouse CD45R conjugated to PE (clone RA3-6B2) |
eBioscience |
Cat#12-0452-83 |
| Anti-mouse CD8a conjugated to PE (clone 53-6.7) |
eBioscience |
Cat#12-0081-85 |
| Anti-mouse Ly-6G conjugated to PE (clone RB6-8C5) |
eBioscience |
Cat#12-5931-83 |
| Anti-mouse TER-119 conjugated to PE (clone TER-119) |
eBioscience |
Cat#12-5921-83 |
| Anti-mouse Ly-6A/E (Sca-1) conjugated to PE-Cy7 (clone D7) |
eBioscience |
Cat#25-5981-82 |
| Anti-c-Myc-Alexa Fluor 488 |
Cell Signaling Technology |
Cat#2279S |
| Anti-AKT (pS473) conjugated to Alexa Fluor 488 (clone M89-61) |
BD Biosciences |
Cat#560404 |
| Anti-P-p38 (T180/Y182) conjugated to Alexa Fluor 647 (clone 28B10) |
Cell Signaling Technology |
Cat#4552S |
| Anti-pERK1/2 (T202/Y204) conjugated to PerCP-eFlour 710 (clone MILAN8R) |
eBioscience |
Cat#46-9109-42 |
| Anti-mouse FcεRIα conjugated to Pacific Blue (clone MAR-1) |
BioLegend |
Cat#134313 |
| Anti-mouse CD49b conjugated to FITC (clone DX5) |
BioLegend |
Cat#108906 |
| Anti-human IgE polyclonal |
Bethyl Laboratories |
Cat#A80-109A |
| Biological Samples |
| Human PBMCs |
Stanford Blood Center |
N/A |
| Mouse C57BL/6J bone marrow cells |
This paper |
N/A |
| Mouse C57BL/6J peritoneal cells |
This paper |
N/A |
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| Chemicals, Peptides, and Recombinant Proteins |
| Recombinant Mouse c-Kit extracellular domains 1-3 protein |
This paper |
N/A |
| Recombinant Human c-Kit extracellular domains 1-3 protein |
This paper |
N/A |
| Recombinant Mouse c-Kit extracellular domains 1-3- Biotin protein |
This paper |
N/A |
| Recombinant Human c-Kit extracellular domains 1-3-Biotin protein |
This paper |
N/A |
| Recombinant Mouse SCF: wild-type protein |
This paper |
N/A |
| Recombinant Human SCF: wild-type protein |
This paper |
N/A |
| Recombinant Mouse SCF: SCFa (with wild-type c-Kit interface and F63A monomer mutation) protein |
This paper |
N/A |
| Recombinant Mouse SCF: S4-3 (with high-affinity c-Kit interface) protein |
This paper |
N/A |
| Recombinant Mouse SCF: S4-3a (with high-affinity c-Kit interface and F63A monomer mutation) protein |
This paper |
N/A |
| Recombinant streptavidin conjugated to Alexa 647 |
In house |
N/A |
| Recombinant Human IL-6 |
Peprotech |
Cat#200-06 |
| Recombinant Human IL-3 |
Peprotech |
Cat#200-03 |
| Recombinant Murine IL-3 |
Peprotech |
Cat#213-13 |
| Recombinant Human IgE |
Millipore |
Cat#AG30P |
| Recombinant anti-GFP nanobody |
(Kirchhofer et al., 2010) |
N/A |
| DY647 maleimide |
Dyomics |
Cat#647-03 |
| Rho11-maleimide |
ATTO-TEC |
Cat#AD Rho11-41 |
| Isopropyl β-D-1-thiogalactopyranoside (IPTG) |
Research Products International Corp. |
Cat#I56000-100.0 |
| Urea |
Fisher Scientific |
Cat#BP169-212 |
| Sodium acetate |
Fisher Scientific |
Cat#BP333-1 |
| Ethylenediamine tetraacetic acid (EDTA) |
Fisher Scientific |
Cat#BP120-500 |
| Dithiothreitol (DTT) |
Sigma-Aldrich |
Cat#D0632-25G |
| Tris-HCl |
Fisher Scientific |
Cat#BP153-1 |
| Oxidized glutathione |
Sigma-Aldrich |
Cat#G4376-10G |
| Reduced glutathione |
Sigma-Aldrich |
Cat#G4251-50G |
| Phenylmethanesulfonylfluoride (PMSF) |
Sigma-Aldrich |
Cat#P7626-1G |
| Ammonium sulfate |
Sigma-Aldrich |
Cat#A4915-5KG |
| Sodium chloride |
Fisher Scientific |
Cat#S271-3 |
| Triton X-114 |
Sigma_Aldrich |
Cat#X114-100ML |
| Triton X-100 |
Sigma-Aldrich |
Cat#T9284-500ML |
| L-Glutamine |
Invitrogen |
Cat#25030081 |
| 2-mercaptoethanol |
Invitrogen |
Cat#21985023 |
| Rat T-STIM |
BD Biosciences |
Cat#354115 |
| Methanol |
Fisher Scientific |
Cat#A412P-4 |
| Paraformaldehyde |
Electron Microscopy Sciences |
Cat#15710 |
| Sodium pyruvate |
Invitrogen |
Cat#11360070 |
| Insulin-transferrin selenium |
Invitrogen |
Cat#41400045 |
| Ciprofloxacin |
Sigma-Aldrich |
Cat#17850-5G-F |
| P-nitrophenyl N-acetyl-β-D-glucosaminide |
Sigma-Aldrich |
Cat#N9376-100MG |
| May-Grünwald stain |
Sigma-Aldrich |
Cat#MG500-500ML |
| Modified Giemsa stain |
Sigma-Aldrich |
Cat#GS500-500ML |
| Evans blue dye |
Sigma-Aldrich |
Cat#E2129 |
| Toluidine Blue O |
Fisher Scientific |
Cat#T161-25 |
| Dimethyl sulfoxide (DMSO) |
Sigma-Aldrich |
Cat#D8418-50ML |
| Methocult basic medium |
STEMCELL Technology |
Cat#M3231 |
| Antibiotic-Antimycotic |
Invitrogen |
Cat#15240062 |
| Critical Commercial Assays |
| OptEIA™ mouse IL-6 ELISA Set |
BD Biosciences |
Cat#555240 |
| Zymoprep™ Yeast Plasmid Miniprep II Kit |
Zymo Research |
Cat#D2004 |
| Primity Bio Pathway Assay |
Primity Bio |
N/A |
| Lineage Cell Depletion Kit |
Miltenyi Biotech |
Cat#130-090-858 |
| ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit |
Genescript |
Cat#L00350
|
| EasySep™ Human CD34 Positive Selection Kit |
STEMCELL Technologies |
Cat#18056 |
| Deposited Data |
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| Experimental Models: Cell Lines |
| Bacteria: E. coli BL21 (DE3) |
Novagen |
Cat#69450 |
| Bacteria: E. coli Rosetta |
In house |
|
| Bacteria: E. coli DH5α |
In house |
N/A |
| Yeast: S. cerevisiae EBY100 |
ATCC |
Cat#MYA-4941 |
| Yeast: S. cerevisiae EBY100 expressing mouse SCF |
This paper |
N/A |
| Yeast: S. cerevisiae EBY100 expressing human SCF |
This paper |
N/A |
| Insect: Trichoplusia ni (High Five) |
Invitrogen |
Cat#B85502
|
| Mouse: MC/9 |
ATCC |
Cat#CRL-8306 |
| Mouse: Bone marrow-cultured mast cells (BMCMC) |
Kalesnikoff and Galli, 2011 |
N/A |
| Mouse: OP9 |
ATCC |
Cat#CRL-2749 |
| Hamster: CHO transfectants secreting mouse SCF |
Gift from Dr. P. Dubreuil, Marseille, France. |
N/A |
| Human: Peripheral blood-cultured mast cells (PBCMC) |
Gaudenzio et al., 2013; Joulia et al., 2015 |
N/A |
| Human: HeLa |
ATCC |
Cat#CCL-2 |
| Experimental Models: Organisms/Strains |
| Mouse: C57BL/6J |
The Jackson Laboratory |
Cat#000664 |
| Mouse: C57BL/6J Cpa3-Cre+;Mcl-l+/+
|
Lilla et al., 2011 |
N/A |
| Mouse: C57BL/6J Cpa3-Cre+;Mcl-lfl/fl
|
Lilla et al., 2011 |
N/A |
| Mouse: C57BL/6J Mcpt8-Cre+/−;DTA−/−
|
Wada et al., 2010 |
N/A |
| Mouse: C57BL/6J Mcpt8-Cre+/−;DTAfl/−
|
Wada et al., 2010 |
N/A |
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| Recombinant DNA |
| pAcGP67a |
BD Biosciences |
Cat#554756 |
| pAcGP67a_mKitD1-3 |
This paper |
N/A |
| pAcGP67a_hKitD1-3 |
This paper |
N/A |
| pAcGP67a_mKitD1-3-GLNDIFEAQKIEWHE |
This paper |
N/A |
| pAcGP67a_hKitD1-3-GLNDIFEAQKIEWHE |
This paper |
N/A |
| pET28a |
Novagen |
Cat#69864-3 |
| pET28a_mSCF-WT |
This paper |
N/A |
| pET28a_mSCF-F63A (SCFa) |
This paper |
N/A |
| pET28a_mSCF-S4-3 |
This paper |
N/A |
| pET28a_mSCF-S4-3-F63A (S4-3a) |
This paper |
N/A |
| pET21a_anti-GFP nanobody |
Kirchhofer et al., 2010 |
N/A |
| pYAL |
Birnbaum et al., 2014 |
N/A |
| pYAL_mSCF |
This paper |
N/A |
| pYAL_hSCF |
This paper |
N/A |
| pSems-26m |
Covalys Biosciences |
N/A |
| pSems_mXFP-mKit |
This paper |
N/A |
| pSems_mEGFP |
This paper |
N/A |
| pSems_tandem-mEGFP |
This paper |
N/A |
| Sequence-Based Reagents |
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| Software and Algorithms |
| UNICORN™ 5.11 software |
GE Healthcare |
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| Prism 6 |
Graphpad |
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| BIAcore T100 evaluation software version 2.0 |
GE Healthcare |
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| FlowJo v10 software |
Tree Star Inc |
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| Spot software version 5.1 |
Diagnostic Instruments |
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| iQ Live Cell Imaging Software |
Andor Technologies |
|
| MPEG Streamclip |
|
http://www.squared5.com |
| ImageJ |
|
http://rsb.info.nih.gov/ij/index.html |
| Other |
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