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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Dev Biol. 2017 Jun 7;428(1):224–231. doi: 10.1016/j.ydbio.2017.06.006

Figure 2. Notch signaling is not involved in embryonic neural commitment.

Figure 2

(A) Pharmacological inhibition of γ-secretase by DAPT during embryogenesis does not affect the pattern and numbers of GLWamide+ neurons in larvae. (B,C) DAPT treatment during embryogenesis does not affect the pattern of RFamide+ neurons (B) or the expression pattern of Ncol1 (C) in larvae. (D) γ-secretase inhibition leads to a dose dependent loss of mature poly-γ-glutamate positive nematocysts in larvae. (E, F) CRISPR-Cas9 mediated mutagenesis of Notch does not affect the number of GLWamide+ neurons (E) but causes a reduction in the numbers of poly-γ-glutamate+ nematocysts (F). Scale bars: 10 µm in (A, B and D) and 40 µm in (C). Data represents combined counting from 3 independent biological replicates. * Student t-test with p<0.05. ** Student t-test with p<0.0001. *** Mann-Whitney U-test with p<0.05.