Fig 4. Association between inhibition of PDE4 and activity against the parasite for benzoxaborole and catechol inhibitors.
Assays to determine IC50 values were performed in duplicate with the total number of assays performed indicated in parentheses: at a minimum, data from two assays are shown. Descriptions of phenotypes observed (descriptors): R = rounded; O = overactive (and perceived degrees thereof using plus and minus symbols); deg = degeneration; tegument damage = damage to the surface of the worms, dark = worms are darkened; none = no effects observed. Wormassay [102] is a digital camera based assay that detects adult worm-induced changes in the occupation and vacancy of pixels between frames (outputted as an average ± S.D.).