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. 2017 Jul 25;6:e27210. doi: 10.7554/eLife.27210

Table 1.

Sequence of the primers used to amplify template for riboprobe synthesis from cDNA.

DOI: http://dx.doi.org/10.7554/eLife.27210.021

Primer name Sequence
 Igf1 F SP6 GCCGATTTAGGTGACACTATAGAAGTGGATGCTCTTCAGTTCGTG
 Igf1 R T7 GAAATTAATACGACTCACTATAGGGTGTTTTGCAGGTTGCTCAAG
 Fgf18 F SP6 GCCGATTTAGGTGACACTATAGAAGCCGCCTGCACTTGCCTGTG
 Fgf18 R T7 GAAATTAATACGACTCACTATAGGGTGGTTTCTCGCAGTTTCCTC
 Egr1 F GTCTTTCAGACATGACAGCGAC
 Egr1 R SP6 GCGATTTAGGTGACACTATAGGTGTCACACAAAAGGCACCAA
 Lef1 F TGAAGCCTCAACACGAACAG
 Lef1 R SP6 GCGATTTAGGTGACACTATAGTTTCCGAAACAACCGTTTTC
 Hif2a F CACTGAGACACCTGCCACCTC
 Hif2a R SP6 CATTTAGGTGACACTATAGGAGGCACCAGCCACCATG
 Agc1 F CCAGCCTGACAACTTCTTTG
 Agc1 R T7 GTAATACGACTCACTATAGGGGGGCACATTATGGAAGCTC
 Fgf18 coding F GCCGAGGAGAATGTGGACTTCCG
 Fgf18 coding R SP6 GCGATTTAGGTGACACTATAGCTAGCCGGGGTGAGTGGGG
 IL6 F CTCTGGTCTTCTGGAGTACC
 IL6 R T7 CGATGTTAATACGACTCACTATAGGGACCATCTGGCTAGGTAACAG
 Mcp5 F GCTTACTCTTCATCTGCTGC
 Mcp5 R T7 CGATGTTAATACGACTCACTATAGGGCTGGTGAAGTGTTTGCAGG