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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Leuk Lymphoma. 2017 Apr 10;58(11):2705–2716. doi: 10.1080/10428194.2017.1306647

Figure 2.

Figure 2

Activation of the DNA-damage response and inhibition of poly(ADP)ribosylation. Cells were exposed continuously for 24 hrs to drug(s) and analyzed by Western blotting (A, B). Cells were treated with drug(s) for 48 hrs and total cell extracts were assayed for PARP enzymatic activity (C), analyzed by Western blotting for the extent of poly(ADP)ribosylation using total cell extracts (D) or soluble and chromatin-bound nuclear extracts (NE, panel E). Similar cell samples were used to isolate genomic DNA that was analyzed (15 μg) on a 0.8% agarose gel (F). DDR, DNA-damage response; NS, non-specific; other abbreviations are the same as described in Figure 1.