Table 1.
List of primary antibodies, their sources and dilutions
| Antigen | Source/Cat. # | Clone type* | Dilution** |
|---|---|---|---|
|
| |||
| AIF | Cell Signaling/5318 | mAb | 2500 |
| ATF2 | Cell Signaling/9226 | mAb | 2500 |
| ATM | Santa Cruz Biotech/25921 | mAb | 750 |
| CHK2 | Cell Signaling/2662 | pAb | 2500 |
| Cleaved CASPASE 3 | Cell Signaling/9661 | pAb | 2500 |
| Cytochrome c | BD Bioscience #556433 | mAb | 2000 |
| DNA LIGASE I | Gene Tex/GTX102936 | pAb | 3500 |
| DNA LIGASE III | Gene Tex/GTX102888 | pAb | 3000 |
| FANCD2 | Santa Cruz Biotech/20022 | mAb | 700 |
| Histone 3 | Abcam//1791 | pAb | 3000 |
| KAP1 | Bethyl Lab/A300-275 | pAb | 3000 |
| MRE11 | Active&Motif/39220 | pAb | 2500 |
| NBS1 | Cell Signaling/3002 | pAb | 2500 |
| NOXA | Calbiochem/OP180 | mAb | 1500 |
| Nucleoporin | Santa Cruz Biotech/48373 | mAb | 700 |
| PAR | Trevigen/#4336-BPC-100 | pAb | 3500 |
| PARP1 | Santa Cruz Biotech/8007 | mAb | 1000 |
| PNK | Gene Tex/GTX107488 | pAb | 2000 |
| RAD50 | Cell Signaling/3427 | pAb | 2500 |
| RAD51 | Santa Cruz Biotech/6862 | pAb | 1000 |
| RAD51AP1 | Gene Tex/106411 | pAb | 3000 |
| RAD52 | Cell Signaling/3425 | pAb | 2500 |
| P-ATF2 (T71) | Cell Signaling/5112 | mAb | 2500 |
| P-ATM (S1981) | Rockland/200-301-400 | mAb | 2000 |
| P-CHK2 (S19) | Cell Signaling/2666 | pAb | 2500 |
| P-KAP1 (S824) | Cell Signaling/4127 | pAb | 2000 |
| P-MRE11 (S676) | Cell Signaling/4859 | pAb | 2000 |
| P-NBS1 (S343) | Cell Signaling/3001 | pAb | 2000 |
| P-RAD50 (S635) | Cell Signaling/14223 | pAb | 2000 |
| P-SAPK/JNK (Thr183/Tyr185) | Cell Signaling/4668 | pAb | 2000 |
| P-SEK1/MKK4 (S257) | Cell Signaling/4514 | mAb | 2000 |
| SAPK/JNK | Cell Signaling/9258 | pAb | 2500 |
| SEK1/MKK4 | Cell Signaling/9152 | pAb | 2000 |
| Ubiquitin | Cell Signaling/3936 | mAb | 2500 |
| VDAC1 | Gene Tex/GTX114187 | pAb | 3500 |
| XRCC1 | Gene Tex/GTX111712 | pAb | 2500 |
| β-ACTIN | Sigma/A5316 | mAb | 6000 |
| γ-H2AX | EMD Millipore/05-636 | mAb | 3000 |
*
pAb: polyclonal antibody; used anti-rabbit IgG (or anti-goat as indicated - G) for secondary antibody from Bio-Rad Lab
mAb: monoclonal antibody; used anti-mouse IgG for secondary antibody from Bio-Rad Lab
**
Fold dilution in PBS with 0.05% Tween 20