Table I. Key Features of 22 Well-Characterized Human T-ALL Cell Lines.
| CELL LINE | IMMUNOPHENOTYPE | TCR STATUS | TRANSLOCATIONS | ONCOGENE GROUP | CDKN2A | CREBBP | FBXW7 | HRAS | JAK1 | KRAS | MYB | NOTCH1 | NRAS | PTEN | TP53 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ALL-SIL | CD3- CD4+ CD8+ | TCR- | NUP214-ABL1 t(10;14) (q24;q11.2)TRAD-TLX1 |
TLX1 | MUT | WT | MUT | MUT | MUT HD & PEST | WT | |||||
| CCRF-CEM | CD3- CD4+ CD8- CD3+ CD4+ CD8- |
αβ mTCR- iTCRβ+ |
[t(5;14) (q35;q32.2)] BCL11B-(TLX3/NKX2-5) SIL-TAL1 |
TAL1 | MUT | MUT | MUT | MUT | HD MUT, PEST WT/MUT | MUT | MUT | ||||
| D1.1 | CD3+ CD4- CD8- | ||||||||||||||
| DND-41 | CD3+ CD4+ CD8- CD3+ CD4- CD8- |
γδ / βδ / TCR- | [t(5;14) (q35.1;q32.2)] BCL11B-TLX3 | TLX3 | WT/MUT | MUT | WT | MUT | MUT | MUT HD & PEST | MUT | WT/MUT | MUT | ||
| DU.528 | CD3- CD4- CD8- | TCR- | [t(1;14) (p32;q11)]TCRδ-TAL1 [t(1; 14) (p33;q11)] |
TAL1 | MUT | MUT | WT HD & PEST | WT | WT | ||||||
| HPB-ALL | CD3+ CD4+ CD8+ | αβ TCRαβ iTCRβ+ |
[t(5;14) (q35;q32.2)] BCL11B-TLX3 | TLX3 | MUT | MUT | WT/MUT | MUT | MUT | MUT HD & PEST | WT/MUT | MUT | |||
| HSB-2 | CD3- CD4- CD8- | TCR- | SIL-TAL1 [t(1;7) (p34;q34)]LCK-TCRβ |
TAL1 | MUT | MUT | MUT | WT/MUT HD & PEST | MUT | WT | WT | ||||
| JURKAT | CD3+ CD4+ CD8- | αβ TCRαβ iTCRβ+ |
TAL1 | MUT | MUT | MUT | MUT | WT | WT | WT/MUT HD & PEST | MUT | MUT | |||
| KARPAS-45 | CD3-CD4-CD8- | αβ | [t(X;11) (q13;q23)]KMT2A-FOXO4 (aka, MLL-AFX) [t(1;5) (q21;q12.2)] |
WT/MUT | MUT | MUT | MUT | MUT HD | MUT | MUT | |||||
| KOPT-K1 | [t(11;14) (p13;q11)]LMO2-TCRγ | TAL1 | MUT | MUT | WT | WT/MUT | MUT HD & PEST | WT | MUT | ||||||
| LOUCY | CD3+ CD4- CD8- | γδ | [t(16;20) (p12;q13)] | ETP | MUT | WT | WT | MUT | WT/MUT | WT HD & PEST | MUT | MUT | |||
| MOLT-3 | CD3- CD4+ CD8- CD3- CD4+ CD8+ |
TCR- mTCR- iTCRβ+ |
[2der(7)t(7;7) (p15;q11)] |
TAL1 LMO2 |
MUT | MUT HD & PEST | MUT | MUT | WT | ||||||
| MOLT-4 | CD3- CD4+ CD8+ | TCR- | [2der(7)t(7;7) (p15;q11)] | TAL1 | MUT | MUT | WT | MUT | MUT | MUT | MUT HD & PEST | MUT | MUT | WT/MUT | |
| MOLT-14 | CD3+ CD4- CD8- | γδ | [t(X;11) (q25;p13)]LMO2-STAG2 | MUT | MUT | MUT | MUT | MUT | MUT | ||||||
| MOLT-16 | CD3+ CD4- CD8- | αβTCRαβ iTCRαβ+ | [t(8;14) (q24;q11)]TCRα-MYC [t(3;11) (q25;p13)]MO2-MBNL1 |
TAL1 | MUT | WT | MUT | WT/MUT | WT HD & PEST | WT/MUT | WT/MUT | ||||
| P12-ICHIKAWA | CD3- CD4+ CD8+ | TCR- | [del(11) (p13p13)]LMO2 rearrangement | LMO2 | MUT | WT | MUT | MUT | WT | MUT | MUT HDWT PEST | MUT | MUT | ||
| CD3- CD4+ CD8- | |||||||||||||||
| PEER | CD3+ CD4+ CD8- | γδ | [t(5;14) (q35.2;q32.2)] NKX2-5-BCL11B NUP214-ABL1 |
MUT | WT | WT/MUT | MUT | WT/MUT HD & PEST | WT | MUT | |||||
| CD3+ CD4+ CD8+ | |||||||||||||||
| PF-382 | CD3- CD4+ CD8+ | TCR- | TAL1 | MUT | WT | MUT | MUT | WT | MUT HD & PEST | MUT | MUT | MUT | |||
| RPMI-8402 | CD3- CD4- CD8- | TCR- | SIL-TAL1 [t(11;14) (p15;q11)]LMO1-TRAD |
TAL1 LMO1 | MUT | MUT | MUT | MUT | MUT | MUT | MUT HD WT PEST | MUT | WT/MUT | ||
| SKW-3 (KE-37) * | CD3- CD4+ CD8+ CD3- CD4+ CD8- |
TCR- | [t(8;14) (q24;q11)]TCRα-MYC | MUT | MUT | WT HD MUT PEST | MUT | ||||||||
| SUP-T1 | CD3- CD4+ CD8+ | β (α non-functional) TCR- mTCR- iTCRβ+ |
[(t7;9)(q34;q34)]TCRß-NOTCH1 [inv(14)(q11;q32)]Igh-TCRα |
MUT | WT | MUT | WT | WT/MUT | MUT | ||||||
| TALL-1 | CD3+ CD4+ CD8+ | αβ | WT | WT HD WT PEST | MUT | WT | MUT |
*
Originally obtained from a T-CLL patient, DNA fingerprint & cytogenetic analyses show some SKW-3 isolates are contaminated or replaced by KE-37, a T-ALL line.
WT: wildtype; MUT: mutated; m: membrane surface; i: intracellular