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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Gastroenterology. 2017 Apr 21;153(2):521–535.e20. doi: 10.1053/j.gastro.2017.04.020

Figure 4.

Figure 4

ICCs, ICC-SCs and gastric muscles are resistant to hyperglycemia-induced apoptosis. (A–C) Apoptosis was quantified by measuring activation of the effector caspases 3 and 7 using Caspase-Glo 3/7 assay (A) or by detecting cleaved caspase 3 (Cl.CASP3) by WB (B–C) in the conditionally immortalized ICC line ICL2A, the wild-type ICC-SC line 2XSCS2F10 and in gastric organotypic cultures (OTC) prepared from postnatal day (PND) 14–16 murine tunica muscularis. Experiments were performed after 72 h of culturing in the presence of various concentrations of glucose. Positive controls were exposed to staurosporine (3 μM; n=3–7/group). Apoptosis was only induced by 4,500 mg/dL glucose in ICL2A cells (*, P<0.05 vs. 100 mg/dL glucose). Red arrows indicate 17- and 19-kDa cleaved caspase 3.