Figure 4. Lymphotoxin-β receptor signaling is required for naïve lymphocyte accumulation.

(A, B) IL-4Rα deficient and WT BALB/c 4get mice were infected with Hp or remained naïve and the number of naïve (A) or IL-4/GFP⁺ effector (B) CD4⁺ T cells in the mesLN was analyzed at 2 wk post-infection. IFNAR KO mice (C) or TNF-α KO mice (D) as well as C57BL/6 WT controls were infected with Hp or remained naive. The number of naive phenotype CD4⁺ T cells in the mesLN was determined 2 wk later. (E, F) BALB/c 4get mice were infected with Hp and LTβR-Ig or control IgG was administered i.p. on days 7 and 10 after infection. The number of naive CD4⁺ T and CD19⁺ B cells (E) or effector phenotype CD4⁺ T cells (F) in the mesLN was analyzed at 2 wk post-infection. (G) B220⁺CD4^- B cells, CD11c⁺MHCII⁺ and CD4⁺B220^- T cells were sorted from the mesLN of 2 week Hp-infected mice and mRNA from each population was assessed by qRT-PCR for the indicated transcripts. Each group shows the mean of 8 individual mice and error bars indicate the SEM. Data are representative of at least two independent experiments. ns, not significant; *p < .05, **p < .01 ***p < .001.