Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 20;67(2):334–347.e5. doi: 10.1016/j.molcel.2017.06.010

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Turnover of Smc Protein Fragments

(A) Equimolar mixtures of BsSmcH-CC8(R558C) and BsSmcH-CC8(N634C) were incubated at 37°C and cross-linked by BMOE at indicated time points. Monomer and cross-linked dimer species were analyzed by electrophoresis and Coomassie staining. A representative image from three replicate experiments is shown.

(B) Quantification of turnover of BsSmcH-CC8 and BsSmcH-CC300 using an Agilent 2100 Bioanalyzer. Cross-linking efficiency is given as fraction of cross-linked protein to total protein. The data points were fitted to an exponential curve. The half-life is given as mean from three replicate experiments. Its standard error (SE) is denoted.

(C) Same as in (A) using BsSmcH-CC300(R558C) and BsSmcH-CC300(N634C) proteins.

See also Figure S1.