Turnover of Smc Protein Fragments
(A) Equimolar mixtures of BsSmcH-CC8(R558C) and BsSmcH-CC8(N634C) were incubated at 37°C and cross-linked by BMOE at indicated time points. Monomer and cross-linked dimer species were analyzed by electrophoresis and Coomassie staining. A representative image from three replicate experiments is shown.
(B) Quantification of turnover of BsSmcH-CC8 and BsSmcH-CC300 using an Agilent 2100 Bioanalyzer. Cross-linking efficiency is given as fraction of cross-linked protein to total protein. The data points were fitted to an exponential curve. The half-life is given as mean from three replicate experiments. Its standard error (SE) is denoted.
(C) Same as in (A) using BsSmcH-CC300(R558C) and BsSmcH-CC300(N634C) proteins.
See also Figure S1.