Figure 4.
Construction of an Archaeal Smc Rod Model
(A) Schematic overview and cartoon representation of several Smc coiled-coil crystal structures used for Smc rod reconstruction. PfSmcH-CC60 (PDB: 4RSJ) partially overlaps with PySmcCC3 (Figure S4A). BsSmcCC2 was transformed into a Py Smc model by sequence substitution. The product overlaps partially with PySmcCC3 at its top and with PySmcHd-CC80 at the bottom. All residue numbers correspond to the Py Smc sequence. Color coding as in Figure 2B.
(B) Reconstructed Py Smc rod in front and side views. Please note the angle in the otherwise straight Smc arm at the Smc joint (right). Color coding as in Figure 2B. The dashed line indicates the symmetry axis (“C2”) of the Smc rod dimer. Triangles in light and dark blue colors denote interfaces at N- and C-terminal Smc residues, respectively (as in Figure 2A). The coordinates of full-length Smc are available in supplemental material (Table S6).
(C) Mapping of Bs Smc cross-linking onto the Py Smc rod model. Color coding as in Figure 2B using Bs and Py Smc sequence comparisons (Figures S3D and S4C).