Antibodies |
|
Anti-Smc polyclonal rabbit antibody, affinity purified |
Gruber Lab |
COD008 |
|
Chemicals, Peptides, and Recombinant Proteins |
|
Adenosine 5′-[g-thio]triphosphate |
Sigma-Aldrich |
Cat #A1388 |
Benzonase |
Sigma-Aldrich |
Cat #A1014 |
Bis(maleimido)ethane (BMOE) |
Thermo Scientific |
Cat #22323 |
Breathe-Easy |
Diversified Biotech |
Cat #BEM-1 |
BsaI |
New England Biolabs |
Cat #R0535L |
Dynabeads Protein G |
Life Technologies |
Cat #10004D |
Erythromycin |
AppliChem |
Cat #A2275 |
GlycoBlue |
Ambion |
Cat #AM9515 |
HaloTag TMR Ligand |
Promega |
Cat #G825A |
His-Tev protease |
MPIB Core Facility |
His-Tev |
HisTrap 5 mL |
GE Healthcare |
Cat #17-5247-01 |
HiTrap Q HP |
GE Healthcare |
Cat #17-1153-01 |
HiLoad 16/60 S200 |
GE Healthcare |
Cat #28989335 |
HiLoad 26/60 S200 |
GE Healthcare |
Cat #28989336 |
HisPur Cobalt Resin |
Thermo Scientific |
Cat #89966 |
Lincomycin |
AppliChem |
Cat #A7697 |
L-Selenomethionine |
Merck |
Cat #561505 |
Phusion Hot Start II DNA Polymerase |
Thermo Scientific |
Cat #F-549L |
Phytic acid |
Sigma-Aldrich |
Cat #68388 |
Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat #P8849 |
Ready-Lyse Lysozyme Solution |
Epicenter |
Cat #R1810M |
RNase A |
Sigma-Aldrich |
Cat #R5125 |
SmDNase |
MPIB Core Facility |
SmDNase |
T4 DNA ligase |
Thermo Scientific |
Cat #EL0016 |
|
Critical Commercial Assays |
|
MasterBlock 96 well, 2 mL |
Greiner Bio One |
Cat #780270 |
Takyon No ROX SYBR MasterMix blue dTTP |
Eurogentec |
Cat #UF-NSMT-B0702 |
NucleoFast 96 PCR Plate |
Macherey Nagel |
Cat #743100.1 |
NuPAGE 4%–12% Bis-Tris Gels |
Life Technologies |
Cat #NP0323BOX |
NuPAGE 3%–8% Tris-Acetate Gels |
Life Technologies |
Cat #EA03755BOX |
QIAquick PCR Purification Kit |
QIAGEN |
Cat #28106 |
|
Deposited Data |
|
BsSmcCC2 |
Protein Data Bank |
PDB: 5NNV
|
BsSmcCC1 |
Protein Data Bank |
PDB: 5NMO
|
PySmcHd-CC80 |
Protein Data Bank |
PDB: 5XEI
|
PySmcCC3 |
Protein Data Bank |
PDB: 5XG2
|
BsSmcHd(EQ)-CC30:ATP-ScpA(C) |
Protein Data Bank |
PDB: 5XG3
|
PfSmcHd-CC25-ScpA(C) |
Protein Data Bank |
PDB: 5XNS
|
Original data |
Mendeley Data |
http://dx.doi.org/10.17632/c2nmr3yhnp.1 |
|
Experimental Models: Organisms/Strains |
|
E. coli and B. subtilis strains, see Table S7
|
Gruber Lab |
N/A |
|
Oligonucleotides |
|
qPCR primers, see Table S3
|
Gruber Lab |
N/A |
PCR primers for HTP cysteine cross-linking screen, see Table S4
|
This paper |
N/A |
|
Recombinant DNA |
|
Plasmid DNA, see Table S8
|
Gruber Lab |
N/A |
|
Software and Algorithms |
|
SHELX |
Sheldrick, 2010 |
http://shelx.uni-ac.gwdg.de/SHELX/ |
XDS |
Kabsch, 2010 |
http://xds.mpimf-heidelberg.mpg.de/ |
Phenix |
Adams et al., 2010 |
https://www.phenix-online.org/ |
CNS |
Brünger et al., 1998 |
http://structure.usc.edu/cns/about_cns/frame.html |
Coot |
Emsley et al., 2010 |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
AMBER 14 |
Case et al., 2014 |
http://ambermd.org/ |
ImageJ |
Schneider et al., 2012 |
https://imagej.nih.gov/ij/ |
Real Time PCR Miner |
Zhao and Fernald, 2005 |
http://ewindup.info/miner/ |
|
Other |
|
pRSFDuet-CPD |
Oh Lab |
N/A |
pJK-CPD |
Oh Lab |
N/A |