REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Smc polyclonal rabbit antibody, affinity purified	Gruber Lab	COD008
Chemicals, Peptides, and Recombinant Proteins
Adenosine 5′-[g-thio]triphosphate	Sigma-Aldrich	Cat #A1388
Benzonase	Sigma-Aldrich	Cat #A1014
Bis(maleimido)ethane (BMOE)	Thermo Scientific	Cat #22323
Breathe-Easy	Diversified Biotech	Cat #BEM-1
BsaI	New England Biolabs	Cat #R0535L
Dynabeads Protein G	Life Technologies	Cat #10004D
Erythromycin	AppliChem	Cat #A2275
GlycoBlue	Ambion	Cat #AM9515
HaloTag TMR Ligand	Promega	Cat #G825A
His-Tev protease	MPIB Core Facility	His-Tev
HisTrap 5 mL	GE Healthcare	Cat #17-5247-01
HiTrap Q HP	GE Healthcare	Cat #17-1153-01
HiLoad 16/60 S200	GE Healthcare	Cat #28989335
HiLoad 26/60 S200	GE Healthcare	Cat #28989336
HisPur Cobalt Resin	Thermo Scientific	Cat #89966
Lincomycin	AppliChem	Cat #A7697
L-Selenomethionine	Merck	Cat #561505
Phusion Hot Start II DNA Polymerase	Thermo Scientific	Cat #F-549L
Phytic acid	Sigma-Aldrich	Cat #68388
Protease Inhibitor Cocktail	Sigma-Aldrich	Cat #P8849
Ready-Lyse Lysozyme Solution	Epicenter	Cat #R1810M
RNase A	Sigma-Aldrich	Cat #R5125
SmDNase	MPIB Core Facility	SmDNase
T4 DNA ligase	Thermo Scientific	Cat #EL0016
Critical Commercial Assays
MasterBlock 96 well, 2 mL	Greiner Bio One	Cat #780270
Takyon No ROX SYBR MasterMix blue dTTP	Eurogentec	Cat #UF-NSMT-B0702
NucleoFast 96 PCR Plate	Macherey Nagel	Cat #743100.1
NuPAGE 4%–12% Bis-Tris Gels	Life Technologies	Cat #NP0323BOX
NuPAGE 3%–8% Tris-Acetate Gels	Life Technologies	Cat #EA03755BOX
QIAquick PCR Purification Kit	QIAGEN	Cat #28106
Deposited Data
BsSmcCC2	Protein Data Bank	PDB: 5NNV
BsSmcCC1	Protein Data Bank	PDB: 5NMO
PySmcHd-CC80	Protein Data Bank	PDB: 5XEI
PySmcCC3	Protein Data Bank	PDB: 5XG2
BsSmcHd(EQ)-CC30:ATP-ScpA(C)	Protein Data Bank	PDB: 5XG3
PfSmcHd-CC25-ScpA(C)	Protein Data Bank	PDB: 5XNS
Original data	Mendeley Data	http://dx.doi.org/10.17632/c2nmr3yhnp.1
Experimental Models: Organisms/Strains
E. coli and B. subtilis strains, see Table S7	Gruber Lab	N/A
Oligonucleotides
qPCR primers, see Table S3	Gruber Lab	N/A
PCR primers for HTP cysteine cross-linking screen, see Table S4	This paper	N/A
Recombinant DNA
Plasmid DNA, see Table S8	Gruber Lab	N/A
Software and Algorithms
SHELX	Sheldrick, 2010	http://shelx.uni-ac.gwdg.de/SHELX/
XDS	Kabsch, 2010	http://xds.mpimf-heidelberg.mpg.de/
Phenix	Adams et al., 2010	https://www.phenix-online.org/
CNS	Brünger et al., 1998	http://structure.usc.edu/cns/about_cns/frame.html
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
AMBER 14	Case et al., 2014	http://ambermd.org/
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
Real Time PCR Miner	Zhao and Fernald, 2005	http://ewindup.info/miner/
Other
pRSFDuet-CPD	Oh Lab	N/A
pJK-CPD	Oh Lab	N/A