Figure 1.
IRC21 Deletion Rescues Checkpoint Mutants
(A) Cells were grown on synthetic-defined (SD/-Ura) plates with glucose 2% or galactose 2% ± hydroxyurea (HU).
(B and C) Cells were grown on YPD plates with or without 5 mM HU (B) or 2 mM HU (C).
(D) sml1Δ, sml1Δ mec1Δ, sml1Δ irc21Δ, and sml1Δ mec1Δ irc21Δ cells were arrested in G1 with α-factor (alpha) and released into YPD containing 0.2 M HU. After 3 hr, cells were released into YPD. Samples were collected at the indicated times to detect Rad53 by western blot analysis.
(E) Cells were arrested with α-factor and released in YPD with or without 0.2 M HU. Cells were treated for 3 hr, and samples were collected to detect Rad53 and Mre11.
See also Figure S1.