Figure 2.

Large Heterochromatin Domains Form upon Removal of mst2⁺

(A) Upper panel: ChIP analysis of H3K9me2 showing enrichments at the target gene ade6-704 and neighboring regions. Error bars indicate SD (n ≥ 3 independent biological replicates). The y axis is shown in logarithmic scale. Middle and lower panels: siRNAs (middle panel) and RNA (lower panel) reads mapping to the ade6-M210 locus and neighboring regions in wild-type (gray), paf1-Q264Stop (red), and paf1-Q264Stop mst2Δ cells (blue), respectively, are shown. Read counts were normalized to the total read number and are depicted in log₂ (middle panel) or linear scale (lower panel).

(B) H3K9me2 enrichments at the right centromere boundary of chromosome 1 (IRC1R). ChIP enrichments are shown relative to the centromeric repeats dg/dh, which was set at 100%. Error bars indicate SD (n = 2 or 3 independent biological replicates; mst2Δ ago1Δ and mst2Δ dcr1Δ or wild-type (WT) and mst2Δ, respectively).

(C) siRNAs mapping to IRC1R and neighboring regions in wild-type (gray), paf1-Q264Stop (red), and paf1-Q264Stop mst2Δ cells (blue). Read counts were normalized to the total read number and are depicted in log₂ scale.

See also Figure S1.