Figure 3.

Mst2 Is Excluded from Constitutive Heterochromatin and Specifically Associates with Transcriptionally Active Chromatin

(A) Mst2 DamID maps for all three chromosomes (bottom) compared to previously generated H3K9me2 ChIP-sequencing data (top; Keller et al., 2013). The signal of Dam-Mst2 (normalized to Dam only) was averaged over 500 probes and is shown in log₂ scale. The x axis shows position on chromosomes.

(B) Enrichment of Dam-Mst2 at different genomic regions. Two independent replicates are shown (scale in log₂).

(C) Zoom of DamID map from (A) on centromere of chromosome 1.

(D) Enrichment of Dam-Mst2 at different euchromatic elements. Two independent replicates are shown (scale in log₂).

(E and F) ChIP enrichment of Mst2-FLAG (blue) compared to untagged Mst2 (black) over the mto1⁺/tef3⁺ locus (E) and the ade6-704 locus (F). ChIP data are shown relative to the mean of the untagged control with the background subtracted (n = 4 ± SEM).