Figure 6.

PCM1 Regulates GABARAP Proteasomal Degradation

(A) Control or PCM1 knockout RPE-1 cells were subjected to cycloheximide (CHX) treatment for the indicated number of hours prior to immunoblotting.

(B) Quantification of (A). Mean ± SEM; n = 3; unpaired Student’s t test; ^∗p ≤ 0.05.

(C) HEK293A cells treated with RF or PCM1 siRNA were incubated in DMSO, cycloheximide (CHX), MG132 (MG), and/or bafilomycin A1 (BAFA1) for 8 hr.

(D) Quantification of (C). Mean ± SEM; n = 3; one-way ANOVA; ^∗p ≤ 0.05.