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Epi-fluorescence micrographs of biofilms developed by P. aeruginosa. The effect of the bacterial extract (0.1 mg/ml) on the viability of reference P. aeruginosa strains in the biofilm was examined at different time points (24, 48, and 72 h) and compared with control. The dead bacterial cells were labeled with propidium iodide whereas live cells stained with SYTO 9, which produced red and green fluorescence, respectively.
