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. 2017 Jul 26;7:340. doi: 10.3389/fcimb.2017.00340

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Copyright © 2017 da Silva, Gomes, Franco, Pereira, Milian, Ribeiro, Fiorenzani, dos Santos, Mineo, da Silva, Ferro and de Freitas Barbosa.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokines production in human villous explants treated with different drugs and infected by T. gondii. Villous explants were collected and cultured during 24 h, infected or not with T. gondii (RH-2F1 clone), and after 24 h were treated or not with the different drugs (μg/mL): enrofloxacin (ENF), toltrazuril (TOL) or combination of sulfadiazine and pyrimethamine (SDZ+PYR) for additional 24 h.The supernatants were collected for detection of IL-6 (A,B) and MIF (C,D). Data were expressed in pg/μg according to the protein concentration of each villous and the standard curve. Data were expressed as mean ± SEM of three independent experiments in nine replicates. Significant difference in relation to untreated and uninfected villous (medium) (^#P < 0.05), untreated and infected villous (T. gondii) (^*P < 0.05), and ENF (^&P < 0.05). Differences between groups were analyzed by One-Way ANOVA with the Bonferroni multiple comparison post-hoc test.