Figure 9.
Ultra-structural analyze of tachyzoites in BeWo cells treated with enrofloxacin or toltrazuril. BeWo cells were cultured in 24-well plates (5 × 105 cells/well/200 μL) for 24 h, infected with T. gondii (RH-2F1 clone) for 3 h, washed with medium to remove extracellular parasites and treated (μg/mL) or not with enrofloxacin or toltrazuril for an additional 24 or 48 h.Then, the cells were submitted to transmission electron microscopy. Representative electromicrography of untreated BeWo cells infected by T. gondii by 24 (A) or 48 h (B), enrofloxacin-treated cells infected by T. gondii by 24 (C) or 48 h (D), and toltrazuril-treated cells infected by T. gondii by 24 (E) or 48 h (F). PV, Parasitophorous vacuole; DC, daughter cells; MC, mother cell; HCN, host cell nucleus; Rp, rhoptries; PN, nucleus; and M, mitochondria of the parasites. Arrows: basal ends (“tethered” parasites); arrowhead: duple membrane. Bar scale: 500 nm or 1.0 μm.