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. 2017 Jul 25;7:6417. doi: 10.1038/s41598-017-06744-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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NPF5.11, NPF5.12 and NPF5.16 are preferentially expressed in vascular tissues. Histochemical localization of GUS activity in NPF5.11 _pro::GUS transgenic plants (a–d), NPF5.12 _pro::GUS transgenic plants (e–h) and NPF5.16 _pro::GUS transgenic plants (i–l). The expression patterns of NPF5.11, NPF5.12 and NPF5.16 were determined in whole-mount seedlings (a,e,i), seedling roots (b,f,j) or cross-sectioned seedling roots (c,d,g,h,k,l). (m,n,o) Transcript expression of NPF5.11 (m), NPF5.12 (n) and NPF5.16 (o) in 28 d old plants. 1–8 indicated leaf positions arranged according to leaf ages (old to young); R, root; F, flower; S, stem. Data were normalized to that of SAND. Values are means ± SD, n = 3. Bars = 10 μm.