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. 2017 Jul 25;7:6349. doi: 10.1038/s41598-017-06484-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of α-SMA and CD68 is decreased in mice with TLR4 deficiency. (A) Representative immunofluorescence image for fibroblast marker, α-SMA and CD68, a marker for inflammatory macrophage. The α-SMA (yellow arrows) and CD68 (white arrows) expression is increased in tubulo-interstitial areas in mice with normal TLR4 to Ang-II treatment. (B) Data is presented as mean fluorescent intensity ± SEM. n = 5/group, tested by Kruskal-Wallis test and Mann-Whitney rank sum test. Magnification ×60. Scale bar: 20 µm. *p < 0.05 vs. C3HeJ + saline, ^†p < 0.05 vs. C3HeOuJ + Ang-II, ^‡p < 0.05 vs. C3HeOuJ + saline. C3HeOuJ: normal TLR4, C3HeJ: TLR4 deficiency.