Figure 7.

The enzyme activity of MnSOD, CuSOD, catalase and the levels of ATP are decreased following Ang-II treatment in mice with normal TLR4. (A) Representative images of gels stained for MnSOD and CuSOD activity. Hundred micrograms of protein were separated in 12% native gels as described in the Materials and Methods. (B) Data shows fold change of mean intensity ± SEM, n = 6/group. (C) The residual amount of H₂O₂ was used as a surrogate for catalase activity. Data shows catalase activity (µmol of H₂O₂ used/min/mL) as mean ± SEM. (D) Data showing ATP levels in all groups as mean fluorescent intensity ± SEM. n = 5/group, tested by Kruskal-Wallis test and Mann-Whitney rank sum test. *p < 0.05 vs. C3HeJ + saline, ^†p < 0.05 vs. C3HeOuJ + Ang-II, ^‡p < 0.05 vs. C3HeOuJ + saline. C3HeOuJ: normal TLR4, C3HeJ: TLR4 deficiency.