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. 2017 Jul 25;7:6427. doi: 10.1038/s41598-017-06817-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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7 YY1 binding sites localized within the Peg3-DMR. (A) The 4-kb genomic region corresponding to the Peg3-DMR is indicated with a dotted line with arrows. The 1^st and 2^nd exons of both Peg3 and Usp29 are indicated with thick vertical lines, whereas the 2.5-kb YY1 binding region is indicated with an open box. The relative positions of the 7 YY1 binding sites are indicated with circles, and the positions of several sets of BiS (Bisulfite Sequencing) primers for DNA methylation analyses are indicated with arrows. (B) The sequence of the 7 YY1 binding sites are shown along with the flanking sequences. The sequences of the 7 YY1 binding sites with red are identical to each other. The three bases of each YY1 binding site were mutated from 5′-GCC-3′ to 5′-ATT-3′.