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. 2017 Jul 25;7:6374. doi: 10.1038/s41598-017-06696-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Reactive microglia immunohistochemistry on HIE brains harvested 48 hours after surgery. Reactive microglia was detected by conducting a CD68 immunohistochemistry on the variable HIE brains. (A) Severe brains showed reactive microglia within the cortex and the hippocampus of the right lesioned hemisphere. CD68 positive cells were also found within the (B) moderate and (C) mild right hemisphere. Whereas CD68 expression was, absent from the (D) normal brain. The brain section images were taken at ×5 magnification and the left and right hemisphere images were taken at ×40 magnification.