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. 2017 Jul 25;7:6382. doi: 10.1038/s41598-017-05412-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-200b is required for normal lung function. (a) Lung mechanics studies performed on 8-week-old mice demonstrated that miR-200b^−/− (ko) mice have higher lung tissue resistance (damping) when challenged with 6 mg/ml methacholine or higher (b) miR-200b ko mice have higher lung tissue stiffness (Elastance) at 12, 25 and 50 mg/ml of methacholine (c) and more conducting airway resistance at high concentrations of methacholine. (d) Accessing lung hysteresivity at the time of saline challenge (before methacholine challenge) showed lower hysteresivity in miR-200b ko mice. *P < 0.05, **P < 0.01, ***P < 0.001, two-way ANOVA. Data represent mean ± SEM of at least six independent experiments. (e) Biophysical surfactant function of miR-200b ko lungs (8-week-old) measured using capillary surfactometry. MiR-200b^−/− mice have decreased surfactant function compared to wt. **P < 0.01, Student’s t-test, Data represent mean ± SEM of at least three independent experiments. (f,g) Immunofluorescence of 8-week-old wt (f) and ko (g) lungs showed decreased abundance of Surfactant Protein-B (SP-B) in ko lungs. (h,i) Immunofluorescence of 8-week-old wt (h) and ko (i) lungs showed decreased abundance of pro-Surfactant Protein-C (SP-C) in ko lungs.