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. 2017 Jul 25;7:6382. doi: 10.1038/s41598-017-05412-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Next Generation Sequencing and Gene ontology (GO) showed the most affected pathways in the lungs of miR-200b^−/− mice. (a) The heat map diagram shows the results of a two-way hierarchical clustering of RNA transcripts and samples. It includes the 500 genes that have the largest coefficient of variation based on FPKM counts. Each row represents one gene and each column represents one sample. The color represents the relative expression level of a transcript across all samples. The color scale is shown below: red represents an expression level above the mean; green represents an expression level below the mean. (wt samples: 867, 871 and 923; miR-200b ko samples: 875, 878 and 869). (b,c) Pie charts for transcript gene functional analysis conducted for Biological Pathway and Protein class using the PANTHER gene ontology database. (d) GO network generated from the GO terms predicted to be enriched for the Biological process (BP vocabulary). Nodes are colored from red to yellow with the node with the strongest support colored red and nodes with no significant enrichment colored yellow. The five nodes with strongest support are marked with rectangular nodes. (e,f,g) Q-PCR confirmed significantly lower Plunc, Cdh26 and Cyp2a5 mRNA levels in miR-200^−/− (ko) lungs than miR-200^+/+(wt).