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. 2017 Jul 25;7:6484. doi: 10.1038/s41598-017-05916-7

Figure 2.

Figure 2

Toxicity of L-lactate on M. tuberculosis. (a) To analyse the effect of increasing amounts of lactate on Mtb, survival of the Mtb H37Rv wild type strain was determined on 7H10 agar plates supplemented with 10 mM, 25 mM, or 50 mM of lactate. Data represent the mean of three independent experiments; error bars indicate the SEM. (b) To determine whether the ∆lldD2 mutant shows an increased sensitivity towards 10 mM lactate, Mtb H37Rv wild type (black lines) and the ∆lldD2 mutant (grey lines) were grown in liquid culture in the presence of either 10 mM glycerol (dashed lines) or on a mixture of 10 mM lactate and 10 mM glycerol (solid lines). Data represent the mean of three independent experiments; error bars indicate the SEM.