Figure 5.

Comparison of acetate derived ¹³C-carbon flux in Mtb wild type and the ∆pckA mutant, and co-metabolism in Mtb wild type during growth on lactate and acetate. To characterize the acetate derived carbon flux in Mtb, the wild type strain (black bars) and the ∆pckA mutant (striped bars) were grown for two days on medium containing 10 mM glucose and 10 mM [U-¹³C₂]-acetate. The overall ¹³C-enrichments are depicted in (a). The relative fractions (%, y-axis) of ¹³C-labelled isotopologues in the wild type strain (black bars) and in the ∆pckA mutant (striped bars) with a given number of ¹³C-atoms (x-axis) are shown in (b). Data represent the mean of two independent experiments each measured in technical triplicates; error bars indicate the SEM. (c) To analyse co-metabolism of lactate and acetate, Mtb H37Rv was grown in defined minimal medium containing 10 mM lactate and 10 mM acetate. Growth and substrate consumption from the medium were detected for ten days at the indicated time points. Data represent the mean of three independent experiments; error bars indicate the SEM.