Fig. 5.
Knockdown of col12a1a/b inhibits functional axon regeneration. a Cartoon showing strategy used for systemic delivery of Vivo-Morpholino (MO), which acts as cell-permeable translation blocking anti-sense molecules. MOs against col12a1a and col12a1b were co-injected into the cardiac ventricle at 3 dpf, 4 h before lesion. b col12a1a/b MO injection strongly reduces Col XII immunoreactivity in the lesion site compared to control MO injected animals. (t-test: ***P < 0.001). c col12a1a/b MO injection inhibits axon regeneration after lesion (Fischer’s exact test: ***P < 0.001). d col12a1a/b MO injection interferes with functional recovery after lesion (two-way ANOVA: F1,116 = 5.534, p < 0.05; t-test: ***P < 0.001). a–d Lateral views are shown (dorsal is up, rostral is left). BF: brightfield. n.s.: not significant. Scale bars: 200 µm c and 100 µm (b,c). Error bars indicate s.e.m