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. 2017 Jul 25;7:6352. doi: 10.1038/s41598-017-06583-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ion distribution image and transverse profile of propranolol in rat small intestine villi following oral administration. (a) Ion distribution images of propranolol (m/z 260.2) together with two different villi markers; m/z 772.6 represents lamina propria region and m/z 804.6 represents the zone of the epithelial cells of the villi outline. Propranolol is represented by a red monochrome colour scale; scaled to 10% of max intensity, m/z 772.6 is represented in green; scaled to 60% of max intensity, and m/z 804.6 is represented in blue; scaled to 20% of max intensity. Scale bar is 100 µm. (b) The intensities of propranolol (red), m/z 772.6 (green), and m/z 804.6 (blue) are also represented by line graphs.