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. 2017 Jul 25;7:6406. doi: 10.1038/s41598-017-06402-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ultrastructure of hAM differs between placental and reflected region. Epithelial cells of placental hAM display predominantly a columnar appearance covered with microvilli. Gaps between the cells are visible. Reflected hAM-derived epithelial cells are cuboidal and exhibit regular cell morphology, microvilli and longer cell protrusions (arrow) and are present at a high density covering the basal membrane. In this subregion of hAM, secretory vesicles are abundant. Upper row: Representative TEM pictures of amnion-derived epithelial cells, 3500x magnification; lower row: Representative SEM pictures of epithelial side of hAM, scale bars: 100 µm.