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. 2017 Jul 25;7:6406. doi: 10.1038/s41598-017-06402-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of PS-associated proteins in isolated hAE cells. (a) Flow cytometric analysis of surfactant-associated proteins and stem cell markers immediately after hAE cells isolation. Cells show similar expression between both amniotic subregions, whereas in general hAE cells of the reflected region show moderately higher PS-associated protein expression and the placental area higher Oct-4 staining. n = 3–5. (b) Immunofluorescence staining of pro-SP-C and SP-B. The predominant part of hAM-derived epithelial cells exhibit distinct staining of both surfactant proteins in the cytoplasm, comparable in both amniotic subregions. Scale bars: 50 µm. n = 4.