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. 2017 Jul 25;7:6406. doi: 10.1038/s41598-017-06402-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hAM explants actively secrete PS components. (a) SP-D is secreted into cell culture medium by hAM. Conditioned medium of reflected hAM explants shows higher SP-D concentrations (83.4 ± 21.5 ng/mL, n = 5) compared to placental hAM (57.8 ± 16.9 ng/mL, n = 5). As positive control, porcine BALF (34.0 ± 6.7 ng/mL, n = 2) was used. Illustrated are mean + S.D. (b) Ultrastructural analysis of hAM reveals lamellar body-like structures inside the cytoplasm, crucial for PS transportation and secretion. Magnification: 8900x and 18000x.