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. 2017 Jul 25;7:6422. doi: 10.1038/s41598-017-06822-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Copy number variation between male vs female genomes of 15 satDNAs with monomers >60 bp in E. surinamensis. The male/female ratio of a relative copy number is shown on a logarithmic scale. The qPCR of male and female genomic DNA was used to calculate the satDNA dose by a ΔCt method of relative quantification (see Supplementary Table S3). Each satDNA family (Esur) is represented by numbers and colors directly in the images. Note that most satDNAs differ significantly between sexes with males having a higher copy number than females for most repeats studied. The statistical significance for difference in copy number between males and females using chi-squared test was highly significant, p-value < 0.01 for each satDNA.