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. 2017 Jul 25;7:6373. doi: 10.1038/s41598-017-06548-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BMP9-induced osteogenic differentiation of rDFCs. (A) ALP staining intensity was enhanced in BMP9 group when compared with the blank group and GFP group. Bars: 50 μm. (B) ALP activity was also significantly increased on days 3, 5, 7, 9 in BMP9 group when compared with the blank group and GFP group. (C) Calcium deposition was stained by Alizarin red staining. More calcified nodules were found in BMP9 group when compared with the blank group and GFP group. Bars: 50 μm. (D,E and F) the relative expression of ALP, OPN, and Osterix were detected by Real Time-qPCR. *P < 0.05, **P < 0.01, ***P < 0.001, compared with blank group, ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, compared with GFP group.