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. 2017 Jul 25;7:6474. doi: 10.1038/s41598-017-06622-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Transcriptome analysis and Q-PCR of class-I SlHSP genes (17.6, 20.0, 20.1) in wild type tomato during ripening. (a) RPKM values for the indicated SlHSP gene transcripts in tomato during plant growth and development and fruit ripening [root, leaf, bud, flower, 3 fruit developmental stages, mature green, breaker and red ripe (breaker + 10)] were derived from RNA-seq data in the SGN database (Solanum lycopersicum cv. Heinz). (b) Q-PCR analysis of SlHSP (17.6,20.0 and 20.1) genes was performed using total RNA isolated from Solanum lycopersicum cv. Ohio8245 and Ailsa Craig wild type fruits at the indicated ripening stages. A 10-fold diluted cDNA was used for transcript quantification using gene specific primers.