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. 2017 Jul 25;7:6474. doi: 10.1038/s41598-017-06622-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative RT-PCR expression analysis of class-I SlHSP (17.6, 20.0 and 20.1) genes in wild type tomato (Solanum lycopersicum cv. Ohio8245) and ethylene-deficient genotype (ACS2-AS). Fruits at mature green stage from two genotypes (WT - Ohio8245) and ethylene-deficient genotype (ACS2-AS) were treated with ethylene(ETH), 1-MCP (1-MCP), or left in air. Fruit RNA was isolated from three independent biological samples and Q-PCR analysis was carried out as described in the Materials and Methods section. Error bars indicate standard deviation of data from a minimum of three replicates.