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. 2017 Jul 25;7:6428. doi: 10.1038/s41598-017-06940-3

Figure 1.

Figure 1

Identification of a 58 kDa P. acnes surface protein recognized by human fibrinogen. (a) Proteins were separated by 12.5% SDS-PAGE (10 μg per lane) and detected by silver staining. (b) Proteins (50 μg per lane) were incubated with biotinylated human fibrinogen, collagens I, IV, VI and VIII (Fg, ColI, ColIV, Col VI, Col VIII; 0.1 μg/ml) and detected with HRP-streptavidin. Control experiments were performed with HRP-streptavidin alone. Lane 1: molecular weight standards. Lanes 2 and 3: surface proteins extracted at 60 °C in PBS and at 45 °C in 1 M LiCl, respectively. The arrow indicates the position of the 58 kDa protein. (c) Immobilized proteins (0.8 to 50 μg/ml) were probed with biotinylated fibrinogen (0.5 μg/ml). (d) Immobilized proteins (25 μg/ml) were probed with biotinylated fibrinogen (0.1 to 16 μg/ml). Data are presented as the mean ± standard deviation of three independent experiments. Statistical significance is indicated by *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.