Table 1.
Measured and calculated molecular masses for tryptic peptides.
| Position | Mass (Da)b | Sequence | |||
|---|---|---|---|---|---|
| Start-End | Observed | Measured | Calculated | Difference | |
| 58–65 | 844.4641 | 843.4568 | 844.4290 | −0.9722 | R.DAVINDAK.L |
| 68–76 | 813.5099 | 812.5026 | 812.5120 | −0.0093 | R.AAIAGALVK.A |
| 77–90 | 1332.6980 | 1331.6907 | 1331.6834 | 0.0074 | K.AGFSSADAVALAPR.I |
| 77–90 | 1333.1610 | 1332.1537 | 1331.6834 | 0.4704 | K.AGFSSADAVALAPR.I |
| 94–107 | 1618.7115 | 1617.7042 | 1617.8661 | −0.1618 | R.EMAKEGVLLINHHK.L |
| 98–107 | 1159.6641 | 1158.6568 | 1158.6509 | 0.0059 | K.EGVLLINHHK.L |
| 108–123 | 1609.9697 | 1608.9624 | 1609.9767 | −1.0142 | K.LKALIGAQVGLLTDAK.I |
| 110–123 | 1369.8074 | 1368.8001 | 1368.7977 | 0.0025 | K.ALIGAQVGLLTDAK.I |
| 127–136 | 928.5557 | 927.5484 | 927.5389 | 0.0095 | R.AAAAVDLGIK.A |
| 137–154 | 1810.0007 | 1808.9934 | 1809.0148 | −0.0214 | K.ATLAATIIPNALHSAAFK.D |
| 155–168 | 1341.7325 | 1340.7252 | 1340.7300 | −0.0048 | K.DAVVANLVAAGVDK.K |
| 155–169 | 1469.8439 | 1468.8366 | 1468.8249 | 0.0117 | K.DAVVANLVAAGVDKK.L |
| 173–192 | 1834.0825 | 1833.0752 | 1833.0723 | 0.0029 | K.ATAVAIAATALNPALGPIAK.T |
| 193–210 | 1841.0198 | 1840.0125 | 1840.0418 | −0.0292 | K.TEAIKAEIGAQAALLVSR.G |
| 198–21 | 1297.7482 | 1296.7409 | 1297.7354 | −0.9944 | K.AEIGAQAALLVSR.G |
| 198–210 | 1298.7502 | 1297.7429 | 1297.7354 | 0.0076 | K.AEIGAQAALLVSR.G |
| 198–210 | 1299.1985 | 1298.1912 | 1297.7354 | 0.4559 | K.AEIGAQAALLVSR.G |
| 216–225 | 1107.6747 | 1106.6674 | 1106.6560 | 0.0114 | K.KAAIEHIIGR.S |
| 217–225 | 979.5738 | 978.5665 | 978.5610 | 0.0055 | K.AAIEHIIGR.S |
| 217–225 | 979.9589 | 978.9516 | 978.5610 | 0.3906 | K.AAIEHIIGR.S |
| 226–244 | 1903.0289 | 1902.0216 | 1902.0211 | 0.0006 | R.SFDAAVATAIVSSPILNAR.I |
| 226–244 | 1903.5977 | 1902.5904 | 1902.0211 | 0.5694 | R.SFDAAVATAIVSSPILNAR.I |
| 245–251 | 837.5310 | 836.5237 | 836.5232 | 0.0005 | R.IVTHLVR.A |
| 252–256 | 503.2774 | 502.2701 | 502.2751 | −0.0050 | R.AGIDK.S |
| 257–265 | 954.5823 | 953.5750 | 953.5658 | 0.0092 | K.SLAVQIAPR.I |
| 257–265 | 954.9483 | 953.9410 | 953.5658 | 0.3752 | K.SLAVQIAPR.I |
| 266–269 | 516.3015 | 515.2942 | 515.3067 | −0.0125 | R.IIDR.L |
| 273–282 | 1068.5443 | 1067.5370 | 1068.6179 | −1.0809 | K.EPLLALNTAK.L |
| 273–282 | 1069.6302 | 1068.6229 | 1068.6179 | 0.0050 | K.EPLLALNTAK.L |
| 290–299 | 1101.6213 | 1100.6140 | 1100.6077 | 0.0063 | R.QIVDVITADK.A |
| 303–309 | 818.4270 | 817.4197 | 817.4181 | 0.0016 | K.TAEQLEK.E |
| 310–319 | 1112.6273 | 1111.6200 | 1111.6125 | 0.0076 | K.ELPALDDLVK.K |
| 310–320 | 1239.7582 | 1238.7509 | 1239.7074 | −0.9565 | K.ELPALDDLVKK.A |
| 384–416 | 3284.4987 | 3283.4914 | 3283.5396 | −0.0481 | R.SGGHSQGGSGTHYIHHGVAPVLTHSSDLPSTGF.– |
| 384–416 | 3285.3900 | 3284.3827 | 3283.5396 | 0.8432 | R.SGGHSQGGSGTHYIHHGVAPVLTHSSDLPSTGF.– |
aThese peptides, which identified the 58 kDa protein, correspond to 267 of the 416 residues, corresponding to 64% sequence coverage (See also Fig. 2). bMono-isotopic masses.