Table 2.
RT-qPCR primer sequences.
| Gene | Forward | Reverse |
|---|---|---|
| β-Actin | CCGAGCGCGAGATCGT | TGGCCATCTCGTTCTCGAA |
| Aggrecan | CATGAGAGAGGCGAATGGAA | TGATCTCGTAGCGATCTTTCTTCT |
| Alpl (Mm) | CCGATGGCACACCTGCTT | GGAGGCATACGCCATCACAT |
| ALPL (Hs) | CCGTGGCAACTCTATCTTTGG | CAGGCCCATTGCCATACAG |
| Bapx1 | ACCTGGCAGCTTCGCTGAA | AGGTCGGCGGCCATCT |
| Col2a1 (Mm) | TGGGTGTTCTATTTATTTATTGTCTTCCT | GCGTTGGACTCACACCAGTTAGT |
| COL2A1 (Hs) | TGGGTGTTCTATTTATTTATTGTCTTCCT | GCGTTGGACTCACACCAGTTAGT |
| Col10a1 (Mm) | CATGCCTGATGGCTTCATAAA | AAGCAGACACGGGCATACCT |
| COL10A1 (Hs) | ATGATGAATACACCAAAGGCTACCT | ACGCACACCTGGTCATTTTCTG |
| Clb2 | TGCCAAGCTTTCTCTGATGCT | GGGTTCTCCCTGTCCTCGTT |
| GAPDH (Hs) | ACTTTGTGAAGCTCATTTCCTGGTA | GTGGTTTGAGGGCTCTTACTCCTT |
| ITS1 (Mm) | TGGGGGGGTGGATGTCTGGAG | CGAGTGATCCACCGCTAAGAGTCGTA |
| ITS1 (Hs) | TGTGAAACCTTCCGACCCCTCT | CGAGTGATCCACCGCTAAGAGTCGTA |
| Mef2c | GGGCCTCAATGGCTGTGA | CTCAGACTCAGGGCTGTGACCTA |
| Osteocalcin | GCGGCCCTGAGTCTGACA | GCCGGAGTCTGTTCACTACCTT |
| Pop4 | GGACACGCAGCCACAGATG | TGACTGAAATAATAGCACCATGAAGAT |
| Pthrp (Mm) | GTTCAGCAGTGGAGTGTC | GATGGTGGAGGAAGAAACG |
| PTHrP (Hs) | AAGGGCAAGTCCATCCAAGA | CTCGGCGGTGTGTGGATTTC |
| Rmrp (Mm) | ATACGAGGGACATGTTCCTTATCC | TTGGCGGGCTAACAGTGACT |
| RMRP (Hs) | GAGAGTGCCACGTGCATACG | ACGCTTCTTGGCGGACTTT |
| Rpp30 | TCAAAAGACCCCCTGTTAATGTG | GATAGCAGGACCATAGACAAGTTCAA |
| Rpp40 | TGTGTCACTACTTCGATGAACCAA | TGTCTGCAAAGCCTTGAACTGT |
| Runx2 | GACGAGGCAAGAGTTTCACC | GGACCGTCCACTGTCACTTT |
| 5.8S rRNA | CACTCGGCTCGTGCGTCGAT | CGCTCAGACAGGCGTAGCCC |
| 18S rRNA | AGTCCCTGCCCTTTGTACACA | GATCCGAGGGCCTCACTAAAC |
| 28S rRNA | GCCATGGTAATCCTGCTCAGTAC | GCTCCTCAGCCAAGCACATAC |
| Sox9 | AGTACCCGCACCTGCACAAC | TACTTGTAGTCCGGGTGGTCTTTC |
| Viperin | TGCTATCTCCTGCGACAGCTT | CCTTGACCACGGCCAATC |
When not specified, primer sequences were designed for Mus musculus. In table Mus musculus is abbreviated as Mm and Homo sapiens as Hs.