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. 2017 Jul 25;7:6409. doi: 10.1038/s41598-017-06600-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM images showing DT-induced ANF damage. Magnification was 6000×, 20000×, and 50000× in A–D, E and F, and G and H, respectively. The images in the left and right columns are from the control and Cre⁺ groups, respectively. The cross sections of ANFs were in regular round shape (A,C) with relatively even thickness of myelin sheaths, in contrast to the irregular shape and uneven thickness of myelin sheaths in the images from the Cre⁺ group (B,D). The white arrows in (B and D) point to nerve fibers whose myelin sheath was peeling off. Additionally, extremely swollen Schwann cells were seen in the Cre⁺ sample (black arrows in B and D). The images of higher magnification (E–H) show that the myelin sheaths in the Cre⁺ sample is thin (F) and loosely packed (H) as compared with the control sample (E and G).