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. Author manuscript; available in PMC: 2017 Jul 26.
Published in final edited form as: Methods Mol Biol. 2016;1403:819–838. doi: 10.1007/978-1-4939-3387-7_47

Fig. 2.

Fig. 2

Confirmation of plasmid linearization and single IVT mRNA product using gel electrophoresis. (a) Verification of plasmid linearization using 1 % agarose/TAE gel electrophoresis. From left to right: NEB 1 kb DNA ladder, 1 µg undigested ovalbumin in pGEM (see Fig. 1), and 1 µg Spe1-digested ovalbumin in pGEM (expected MW = 4100 bp). (b) Verification of single IVT mRNA product using 1.5 % denaturing agarose gel electrophoresis. From left to right: Invitrogen 0.5–10 kb RNA ladder and 1 µg IVT ovalbumin mRNA (expected MW = 2054 bases)