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. 2017 Jul 25;7:6413. doi: 10.1038/s41598-017-06844-2

Figure 3.

Figure 3

Hyperoside pre-treatment restored the decreased renal HS contents and attenuated GBM thickening in DN mice (n = 5). (A) The glomerular HS expression was determined by immunofluorescence staining. (C) The columns quantified the amount of immuno-reactive HS by NIH Image software. (B) GBM thickness was measured by transmission electron microscopy (×25000). (D) Quantifications of GBM thickness was presented in columns. (E) Western blot analysis of renal cortex HS protein levels and the columns showed the amount of HS protein levels relative to β-actin. Data are presented as mean ± SD. P < 0.05 is statistically significant. aIndicates significant vs. Control, bindicates significant vs. DN.