Figure 3.

eDNA production in Bacillus subtilis 3610. (A) Batch culture of strain 3610 in MSgg at 37°C with aeration. A600 refers to the absorbance of the culture at 600 nm, and eDNA refers to the concentration of eDNA in the culture supernatant. eDNA is released during the transition from exponential to stationary phase, followed by a rapid decrease in eDNA concentration. The data presented are representative of the results obtained in ten different experiments. (B) fluorescent microscopy image of cells from a wild type 3610 strain (1) and an eDNA defective mutant (2) after 5 h 30 min in batch growth. The DNA is stained blue (DAPI) and cellular membranes are stained red (FM4.64). A subpopulation of cells has a greater intensity of DNA staining in a wild type strain but not in a defective mutant in eDNA production.