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. 2017 Jun 30;7(2):299–312. doi: 10.15171/apb.2017.036

Figure 1.

Figure 1

Engineering and verification of pCDNA3.3-scFv 183-H12-5C vector. Schematic representation of expression vector pCDNA3.3-scFv 183-H12-5C (A). scFv 183-H12-5C gene was amplified from pHEN2-scFv 183-H12-5C, restricted and cloned at SfiI/NotI restriction sites in inversely amplification pCDNA3.3 vector (B). Colony PCR used CMV-F/scFv 183-H12-5C NotI-R primers; Presence of ~860 bp band in most of the clones suggests the presence of scFv 183-H12-5C in pCDNA3.3-scFv 183-H12-5C plasmid vector (C). Restriction analysis of pCDNA3.3-scFv 183-H12-5C with SfiI/NotI (Linearized plasmids) (D).