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Engineering and verification of pCDNA3.3-mu-IgGk-scFv 183-H12-5C vector. Schematic representation of expression vector pCDNA3.3-mu-IgGk-scFv 183-H12-5C (A). Colony PCR used BsiWI-muIgGk-F/6His-A-seq-R primers; Presence of ~860 bp band in most of the clones suggests the presence of mu-IgGk-scFv 183-H12-5C in pCDNA3.3-mu-IgGk-scFv 183-H12-5C plasmid vector (B). Restriction analysis of pCDNA3.3-mu-IgGk-scFv 183-H12-5C with BsiWI/NotI (5.4 kb vector backbone + 855 bp insert) (C).
